RNA Abundance Analysis: Methods and Protocols

Hailing Jin (Curatore)

Isgouhi Kaloshian (Curatore)

Springer-Verlag New York Inc., 2021

This updated book covers a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms. RNA editing and the computational data processing for genome-wide datasets is also discussed. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, RNA Abundance Analysis: Methods and Protocols, Second Edition provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and other RNA variants, in order to assist researchers in their gene expression studies.

• Curatore: Hailing Jin, Isgouhi Kaloshian
• Editore: Springer-Verlag New York Inc.
• Collana: Methods in Molecular Biology
• Anno: 2021
• Rilegatura: Paperback / softback
• Pagine: 233 p.
• Dimensioni: 254 x 178 mm
• EAN: 9781071607459

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